FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_ColSPL8_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_ColSPL8_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995159
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1403.288278E-722.498450
TATGCCG1253.0932442E-622.39840948
GCCGTCT1304.346619E-621.5369351
GTATGCC1408.254492E-619.99857947
AACGGCC1000.00969344817.49875629
GTCCCCG1000.00969344817.49875610
ATGCCGT1704.3875945E-516.46941849
GGACTGT1600.00909619713.1247277
CGTATGC2356.8490487E-411.91404646
CTCGTAT2701.6726895E-411.66583844
GAATGCC2600.001596095310.76846538
ACGGATG2650.001870551310.56528756
TGCACTT3055.170935E-410.32713521
GCTATTA2800.00295500629.999289553
TCGTATG3400.0014001879.26404845
CTGCTTG3100.00684181839.03161759
ACCTTGG3150.00780149278.88825745
GATCTCG3750.0034085488.39940441
TCTCGTA4250.00114476358.23470943
TTACTCG4550.00224259577.691760535
Produced by FastQC (version 0.11.5)