FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_ColSPL16_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_ColSPL16_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004342
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1900.025.788149
TATGCCG1951.8189894E-1225.12686348
TGCCGTC2001.8189894E-1224.49869350
GCCGTCT2301.2732926E-1121.30321351
GTATGCC2551.8189894E-1220.58713747
CGTATGC2901.8189894E-1118.10248446
CTCGTAT3351.5825208E-1015.67080644
TCGTATG3452.4556357E-1015.21657945
CATCTCG4001.5097612E-1013.99925341
TCTCGTA4152.7102942E-1013.49325643
CCGTCTT3801.5519618E-812.8940552
ATCTCGT4853.1814125E-911.54577542
CTGCTTG4156.7452856E-710.9632759
CTTCTGC4803.3689503E-810.93691656
CGTCTTC5805.1577445E-89.65465753
AGTCACG5552.813449E-79.45895528
TCACGAG5401.9723884E-69.0735930
CACGAGA5754.6096884E-68.52128531
CCATCTC5754.6096884E-68.52128540
ACGAGAT7402.7222995E-88.5130632
Produced by FastQC (version 0.11.5)