FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_ColSPL13_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_ColSPL13_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605357
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1703.763489E-922.64604248
ATGCCGT2005.156089E-717.49921449
TGCCGTC2005.156089E-717.49921450
GTATGCC2503.1203854E-715.39930847
GCCGTCT2454.2742868E-614.28507251
GCCGATG2200.00567258211.13586269
CGTATGC3206.635376E-510.93700946
CCCGCCT2250.006704805410.8883998
CGCTCAT3903.822319E-610.76874534
CTGCTTG5452.1639062E-79.63259559
CTCGTAT4005.9267382E-59.62456844
GGCATCT3000.005206639.3372766
TCGTATG4502.1369921E-48.55517145
TCTCGTA4956.4297164E-58.484467543
CCGTCTT5957.31024E-68.23492452
CCGCTCA4400.00161344467.95418833
CGTCTTC8158.936795E-66.870857253
ACCGCTC5250.009032316.666366632
CTTCTGC6800.00245311326.17619356
GCTCATT7650.0090755235.48994935
Produced by FastQC (version 0.11.5)