FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_ColSPL11_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_ColSPL11_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779061
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2950.023.72783346
TATGCCG2850.023.33236748
GCCGTCT2650.022.451951
TGCCGTC2850.022.10434750
ATGCCGT3200.020.78039249
GTATGCC3750.017.732647
AGGGGGG2751.3976818E-512.7364111
TCGTATG5053.6379788E-1112.47473145
CCGTCTT5004.0563464E-1011.89950852
CTCGTAT5652.6011548E-1011.149981544
CTGCTTG5158.1581675E-910.87333759
TCTCGTA7604.632966E-98.74963943
ATCTCGT8757.585186E-108.39965342
AATCTCG9503.781679E-97.73652241
CGTCTTC8452.5585723E-77.45531353
CATTCAG12001.2187229E-107.291365633
TCCAGTC13451.0913936E-86.245095370
CAGTCAC11459.175965E-76.113284627
CACATTC13801.8853825E-86.086704731
CTCCAGT14407.2868716E-96.07613769
Produced by FastQC (version 0.11.5)