FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_ColSPL10_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_ColSPL10_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734771
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG4800.023.33247242
CGGGGGT3002.910383E-1117.49935544
CGGGGTG1957.846287E-616.1532553
AGGGGGG4450.015.7401171
GGCCCCG1656.526023E-414.84793913
AATCGGG8100.014.69081641
TCGGGGG5750.013.99948443
GGCCCTG1600.00910248313.124516568
ACTCCGG11350.012.64270632
GCGGGGT2952.8956289E-511.86397152
TCCGGAG12750.011.25448734
TCGGGGT3451.1608678E-511.1590143
GAATCGG11500.010.95611940
CACTCCG12800.010.93709831
CGGGGTT3903.825153E-610.76883344
CTCCGGA13900.010.5751533
CCGGAGA14150.010.3883135
CGGACTA3208.0204516E-49.8469886
GGCGGGG3400.00140138989.26436551
GCTAGAC3050.00599010159.1799922
Produced by FastQC (version 0.11.5)