FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_COLSPL8_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_COLSPL8_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701539
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3100.022.57988751
TATGCCG3450.022.31767848
ATGCCGT3900.019.74256149
GTATGCC4400.019.08991447
CGTATGC4050.018.14720246
TGCCGTC4250.018.11670550
CACGGCC1901.13549526E-414.73580319
CTCGTAT6100.012.62229344
CATCTCG7050.011.41805641
TCGTATG6800.011.3229445
CCCGGCC2200.005675365711.13557725
GTGCGAA2650.001864847710.5703786
CTGCTTG8252.382876E-108.90895759
CCGTCTT8656.548362E-118.90143652
ATCTCGT10001.8189894E-128.74970642
GTCACGA9759.094947E-128.61493529
AACGGCT8655.984475E-108.5034351
TCTCGTA9606.184564E-118.3849843
TCACGAG10657.2759576E-128.21553430
AGTCACG10302.2955646E-97.47533928
Produced by FastQC (version 0.11.5)