FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_COLSPL16_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_COLSPL16_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008166
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1356.0242637E-620.74041748
ATGCCGT1451.1170965E-519.31004549
TGCCGTC1551.984475E-518.06423650
GCCGTCT1755.6205095E-515.9997551
GTATGCC1554.0550093E-415.80620647
CGTATGC1708.17484E-414.4115446
CCGTCTT3257.758613E-510.76906252
CTCGTAT3000.00522260979.33318844
CATCTCG3000.00522260979.33318841
CTTCTGC4200.0094247867.499883756
TGCTTGA5900.00382006776.52532260
CTGAACT10400.00178345725.04824970
Produced by FastQC (version 0.11.5)