FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_COLSPL13_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_COLSPL13_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130250
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3850.029.08956148
CGTATGC4000.028.87366546
GCCGTCT3800.028.5513151
TGCCGTC4150.027.8300450
ATGCCGT4300.026.04530749
GTATGCC4900.023.57033747
CTCGTAT5800.019.30945244
TCGTATG6550.018.70142645
CTGCCGC1000.00969558717.49919142
CTGCTTG7750.015.35448959
CGTCTGG1450.004743121614.48208943
TCTCGTA7550.014.37019543
CCGTCTT7900.013.73354252
CATCTCG8200.013.23109441
CGTCTTC10150.012.06840653
AGGGGGG4004.1646854E-711.3819541
ATCTCGT9950.011.2557642
GTCACGA11350.010.79245229
CTTCTGC11900.010.58774656
TCACGAG12350.010.48534430
Produced by FastQC (version 0.11.5)