FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_COLSPL10_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_COLSPL10_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATATTATAACTCAGGTTATTTTTTTGCTTAACGTAGTTTATACAATA22.4390243902439024No Hit
ACAAATCAAGGTAAATGGTCCAAGTAACAATATAATAATGAATAAGCGAA11.2195121951219512No Hit
TTAAAGAGTATAAATTGACTTACTATACTCCTGAATATGAAACCAAGGAT11.2195121951219512No Hit
AACCGAAACAAGTTCCTTAAGATATATATAACATAAACATATCCTTTATA11.2195121951219512No Hit
CTTATAAAAATAAAAAGAAAATGAGAGATTGAAAGGAATTTCGGATAGTG11.2195121951219512No Hit
CCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTC11.2195121951219512No Hit
AGGAATCGACAGTGGAGAGTGGAGGGAAAAAGAGGTCACTTGAGTAAGAC11.2195121951219512No Hit
AGTACTTAAAGGTCAAAATTTGGAGTCGACAAAAAGTCAATGGAAAAGTT11.2195121951219512No Hit
TGTAAAAAAACTAGAACCGCAACATATCTTAAAAGCATAAGTATTGTATC11.2195121951219512No Hit
GTTACGGATGCGATCATACCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTA11.2195121951219512No Hit
GAACTTGATAAATCTCAAGGCCGTTGATAAGAGGAGGATGAGTAGAGTTA11.2195121951219512No Hit
TAAGAAGAAAATAAAAATAAAAAAAAATAAAAGCAATTTAATTTTAAAAA11.2195121951219512No Hit
GACATTAAGTCATATTCGACTCCAAAACACTAACCAACCTTCTTCTTGCT11.2195121951219512No Hit
AATACTATATTAAAATTTAAATATGATAGACATAATCATTAAATTAGTTC11.2195121951219512No Hit
TAAACCGCAACCCAATCTTGTAAGCCTAAGAACTGTATCCTTGTTAGAAC11.2195121951219512No Hit
GTAGAATATAAAATATTTTTTTAATATTTTTATACCCAAATTCATCATTT11.2195121951219512No Hit
GTACCTCCAATAAAACAGGGAAAGAATGCGGCTTTGTCTGAGACACACAT11.2195121951219512No Hit
GAGTAACATCAACCCCACTCCGTGACTTTGTTTTAAACACACTAGTTGCA11.2195121951219512No Hit
ACAACTTTAACCGCAACCAGATCTTAAAAGCCCAATTAGTGTTTTCTTGT11.2195121951219512No Hit
ACCATTGACCGTAATACTCCCTGACCCTTCTTCTCCAAATTTCAGCTGCC11.2195121951219512No Hit
AATAGAAGAGCAGGCTGCATATAATGAGAAGAGAGATGATGAGATTGCTG11.2195121951219512No Hit
AAACCGTCTCCCACATTTTCAAATTACTGTAATCTATAGCGGCGTTCATA11.2195121951219512No Hit
TACTAACTTATGTAGATCTTAATAAGTTGATGTCCAGTTCTGAATATTGA11.2195121951219512No Hit
AGAAACTCTGTTATAGCCATTTCTGTACATTCAATGTACTCTACGGATAG11.2195121951219512No Hit
ACAAAACCCAATTTGGGAAACTTGTTCTTAATTTTTTTATTTATTTTTGT11.2195121951219512No Hit
CAATAGTGTGTTCGAGGCAGAAAATGCGAGGTCTTATATATCTAGAAGAC11.2195121951219512No Hit
TTTCAGAATTTCTAGGATACCGAAAAAATCACACCTCTACGAAAATGGTA11.2195121951219512No Hit
AAAAGGGTTTAATTCTTGATAAGAACTTCAGTTGGATTATGCTTGTTAAT11.2195121951219512No Hit
TTCTTTTCTATTTCAATTTCTGGATTATTATATGATGATTTTGCAACTTT11.2195121951219512No Hit
CATGGATCCTTTAATTATCTCTCGACGAAAATCAGATTGTTGCGAATAAC11.2195121951219512No Hit
TATATTGGCCAAGGTTAGTGAAATGTTGTTTAAAATAGTATTTCGATGTA11.2195121951219512No Hit
GACTCACCAACATCTTACGATGAGACATCGGGCTACCTGGCAAGAGTCAA11.2195121951219512No Hit
AGCTGGAATTTGCACACTGTCTGAACGAGCTCGGTGTCGATCGACAGGAG11.2195121951219512No Hit
CAAAGCTTCAACATCAAACATCATCCAGAGACAAACAGAGAGAAAGCATT11.2195121951219512No Hit
CATAGTGAAAATTATACTCATTCTACTAAAATAATGACTTCAAATACAAA11.2195121951219512No Hit
CCCCTAGAACAACATTTTCTTTCAGGCCTTTCAACCAATCGATACGACCC11.2195121951219512No Hit
TCATAGAAGAAAGAAGAATCCGTTTAAAAAGTCTATAGCTGGAACTAGAT11.2195121951219512No Hit
CTCCACAACAATCTCTGGCAGCAATCACTCTCTTGTACCTTCAATATCTA11.2195121951219512No Hit
ATCTAAGAGATCCTCAAAACCCTTGCTGTCTGTTTAGGACATCCTTAGAT11.2195121951219512No Hit
CTTCGACTTCCATATGGATACGTATGGAAAGTATAACCGCGAGTGAAGTA11.2195121951219512No Hit
GGTCCACGTTACGGAATATGGAGTACACTGGAATTTCTTTTTCACACTTG11.2195121951219512No Hit
ATAAATATCAGTTTTGTAATTCTAATATTAGAATATTTTAAATTTTCTAT11.2195121951219512No Hit
ACTCGTAAGAAAAATTAACTCGTTAATTTTTTTTGAATATTTTCGTTTTT11.2195121951219512No Hit
GCCCATTTCATGTTCATTAATCATAAATACGTAAAAGAGGTCGCCTGTAT11.2195121951219512No Hit
CGGCGAGCGTTCTCATCTTCTTCTTCTCCTCCGTGTGTCAGCAGTTTCTT11.2195121951219512No Hit
CATTCAAGTTTGACCCTTGGACGAAAGTGATTTGGCCTACAACATCTAGA11.2195121951219512No Hit
AAAAAAATACTCAACCATGATGCATTCGAACTAAGACTTCTCTTGGTTGA11.2195121951219512No Hit
ATTTTATTGTTAATTCCAAAATTTCTTGTTTTATATTAAAACTGGTTTTC11.2195121951219512No Hit
AAACATAACAAAAAACAATGGATCATTTGGTTTACTTAGTTGAGAAATTG11.2195121951219512No Hit
CTAAATGGAATGTAACGACGTAGGGATCCCTATGTAAACATAGTATCTAT11.2195121951219512No Hit
CCGGCTCCAGTCAAGGCCGCAAGAAATATCAAAACACCAACAAAAGAAGC11.2195121951219512No Hit
ACTCACGCTTTGTCATTCACTTATCTTATATCTTATTGATTCCTCGTCGA11.2195121951219512No Hit
AATTTAAAGCCTAACCAAAAGAAAAGAGTAAACGATTTCTGTAAAACGAT11.2195121951219512No Hit
TTTATCATAGGATTGAGATAACGTTTATTTGGAAAATAATTAAAGGTTGT11.2195121951219512No Hit
GTTTGGTTCAGGGGCTTACCGAACCGGATTGAACTTCTCATATACAAAAA11.2195121951219512No Hit
GCGTTCATTTCAAGACACATTTTAAATATTAAGACCACCAAAAAGAGAAA11.2195121951219512No Hit
AAAACAAGGTATAATTGAAACCATTATTTGAAATTAAGTATCAAATATTT11.2195121951219512No Hit
ATATATACAGTCTGGACTCAAATATACTTACTTATATAGACATGCACAAA11.2195121951219512No Hit
TTTCAAGGAAAAAAACAAGATATAACCTTTTATTTCAGGATTTTTATCCA11.2195121951219512No Hit
AGTCAAAAATACCAAGAAGAAGCAATCCTTTGTTTTGGAATTAACCGAAA11.2195121951219512No Hit
ATCATAAAAAACTTCGATTCTTTACAAAAAGAGTTTCTAAAGAACCTTAA11.2195121951219512No Hit
ATCATAAAAAACTTCGCTTCTTTACAAAAAGAGTTTCTAAAGAACCTTAA11.2195121951219512No Hit
AATGCAAAACTAAATGATGATAAATACACACCTAAAATGAGTAAAATACA11.2195121951219512No Hit
AGCCCGCATGATTTCCCTTTGTCCGAGCTTATGGCCAACTCCTTCCCACT11.2195121951219512No Hit
TTTTCAGGAAATTTCCAAACGAACAATTTCAACGAAATCTTTCAATTTCT11.2195121951219512No Hit
TATGTCCTATGTCAAGCTAACCAAGTACGTAGTATACATATGTCATATGT11.2195121951219512No Hit
CTGGTCGTTCTTGATCGTTTGTATCTTTGGTTTCAGATTCACTGTCATTA11.2195121951219512No Hit
GGATGGTTAAGAGATTATCTATGGTTAAACTCTTCACAACTTATCAATGG11.2195121951219512No Hit
TTTTTGTGCAAAAAAAAAGTAAAAGAAGAAAATATTTAGATTATCACCAA11.2195121951219512No Hit
TTTAAGCGTGACCAAACCCGGACCATTATAATTTGGATTTGTTTACCCCA11.2195121951219512No Hit
ATAATTCGAAAATAAAACAGCAAGTACAAAAATGAGTAATAACCCCCAAT11.2195121951219512No Hit
CAAAGGTAGATATTTTTCCCAACGTCCTCCCCAATCCAGCATACAAGCTC11.2195121951219512No Hit
ATACAATTTAATTTACAAAAAAAGTTATTAAATCTAATTCAAAAGTAACT11.2195121951219512No Hit
GGAGAGTAAAAGAAAACTTACTCATAGTGGACAGGAGAAACACCTTGAAA11.2195121951219512No Hit
ACAATGATGGATACACTAAGAGAAGAAGAAACCTAGCAAAAATGAAAATT11.2195121951219512No Hit
AATACCATTCTTTTTAAAAGTTACTGATCAATGGGTTGAGATGAATGTAA11.2195121951219512No Hit
ATGAGGAAATATCCATTAGTGAACTTCAAGACTTGCTGCCTTATCGATGC11.2195121951219512No Hit
AACATTATTTATTAAAATCACATGTAATTTATTCTAAAAAAATGATTTAA11.2195121951219512No Hit
CTTGGCCACTTAGATGTAAGGCCTAGCTCCATCCACCATCTTCAAAAAGA11.2195121951219512No Hit
GTCCAATGTCACTATCTTGCTTTCTTTTATGGGTAATTTATTTAAAGATA11.2195121951219512No Hit
TGCGTGAACTTGGTCCTTTGAAGTATTTCTTGGGTTTGGAGATAGCTCGA11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACA50.070.021
CAAGAGT50.070.041
GGTGAAC50.070.057
TAAATTG50.070.051
TGGCAAG50.070.038
CCAACAT50.070.07
CCTATGA50.070.063
ATCTTAC50.070.012
GCAAGAG50.070.040
AAATTGG50.070.052
GTCAATA50.070.046
CATCTTA50.070.011
GGCAAGA50.070.039
AAGAGTC50.070.042
ATTGGTG50.070.054
CAACATC50.070.08
ACCAACA50.070.06
TATGAGA50.070.065
AACCTAT50.070.061
TCAATAA50.070.047