FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_C24SPL16_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_C24SPL16_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784195
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2850.025.78814150
TATGCCG2750.024.18056948
ATGCCGT3100.021.45050449
GCCGTCT3350.019.8497251
GTATGCC3700.017.97204447
CGTATGC3650.017.25938446
CCGTCTT5153.6379788E-1212.91195552
AGGGGGG2852.0139449E-512.2914341
TCGTATG5004.0563464E-1011.89938545
CTCGTAT4954.3910404E-911.31254744
TCTCGTA6452.382876E-1010.30954543
CGTCTTC7554.116373E-98.80749253
CTGCTTG6151.1381391E-57.967291459
CATTCAG10706.2573235E-107.52297633
ATCTCGT8553.1149466E-77.3680442
AATCTCG8452.02905E-67.041056641
GCTTGAA10452.377601E-87.033326661
GTCACAT12553.3833203E-106.97175129
CACATTC14301.364242E-106.60805131
CAGTCAC12105.705442E-86.36330827
Produced by FastQC (version 0.11.5)