FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_C24SPL15m2_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_C24SPL15m2_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100546
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11750.013.40363350
ATGCCGT11950.013.17930349
GCCGTCT11650.012.91785451
TATGCCG12550.011.99147548
CGTATGC14800.010.404922546
GTATGCC15050.010.23208347
CGGACTG12900.08.6858956
GGACTGT15300.07.7774947
CGCTCAT19500.07.717590334
CCGCTCA21350.07.2127833
TGGGGGG10251.2355486E-76.83219775
ACCGCTC23400.06.58089132
CTCGTAT24000.06.562195344
TCGTATG25200.06.52747445
TCACCGC27100.06.328119830
TACGCCC7206.757904E-46.319150413
CCGTCTT28050.05.989026552
CACCGCT25900.05.945669731
GTCACCG27350.05.63045129
GACTGTA20751.0950316E-95.2286738