FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_C24SPL15m1_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_C24SPL15m1_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4505802
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6550.027.78548
TGCCGTC7500.023.7989250
ATGCCGT7750.023.48280549
GTATGCC8550.022.92293747
GCCGTCT7800.022.88357751
CGTATGC10050.017.41214646
CGCTCAT12450.014.89892334
CCGCTCA12300.014.22699633
ACCGCTC13300.013.15729832
CCGTCTT15550.012.82952
CTCGTAT16700.011.73599544
TCACCGC16050.011.33904930
TCGTATG17000.011.32301645
CACCGCT16400.011.09705731
GTCACCG16250.010.98411829
GCGCGAA2250.006708002610.88839561
CTGCTTG19250.010.18135659
AGGGGGG7655.456968E-1210.0720561
CGTCTTC21850.09.45037353
GCTCATT20450.09.41277535
Produced by FastQC (version 0.11.5)