FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_C24SPL15m1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_C24SPL15m1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594977
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4950.024.7461848
GCCGTCT4300.024.41826851
CGTATGC5400.023.98022846
GTATGCC5400.023.33211547
ATGCCGT5050.022.8700949
TGCCGTC4900.022.14169950
GTGCCCG1000.00969647917.49908433
AGGGGGG4450.014.9542771
CTCGTAT8550.014.32673644
TCGTATG8600.014.24344245
CCGTCTT7800.013.46135252
ATCTCGT12650.011.34328142
TCTCGTA11250.011.19941443
CATTCAG15400.010.6812633
CTGCTTG9650.010.51819359
AATCTCG13550.010.33156341
CGTCTTC11100.010.089951553
CTTCTGC12650.08.85363456
CAGTCAC19050.08.45067627
CACATTC22100.07.759775631
Produced by FastQC (version 0.11.5)