FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_C24SPL11_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_C24SPL11_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217454
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT12750.10472674942954724TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2900.034.99819650
CGTATGC3050.034.42445446
TATGCCG3200.032.81080648
GTATGCC3350.032.38638747
GCCGTCT3200.030.62341951
ATGCCGT3450.030.43321249
TCGTATG4450.023.59428645
CTGCGCC850.003792025620.58717364
CCGTCTT5150.019.70772252
CTCGTAT5400.019.4434444
AGGGGGG2353.7471182E-1019.3766171
CTGCTTG5250.019.33233659
GATCTCG5850.019.1443141
CGATCTC6550.017.09835240
TCTCGTA6400.016.40540343
CGTCTTC6900.015.21660653
CGGCTAG1400.003755382314.99922543
ATCTCGT7100.014.78796842
CTTCTGC7350.014.28497756
TTACTCG8000.012.68684635
Produced by FastQC (version 0.11.5)