FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_C24SPL11_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_C24SPL11_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026828
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3800.031.31391350
TATGCCG4150.031.20295148
ATGCCGT4200.029.16491949
CGTATGC4550.028.45983346
GTATGCC4600.028.15048847
GCCGTCT3950.027.46806551
CCGTCTT6250.017.91981152
TCGTATG7700.016.36265845
CTCGTAT7800.016.15287844
TCTCGTA8700.014.88416643
CTGCTTG7900.014.61973859
ATCTCGT10900.012.52218542
AATCTCG11300.012.388638541
CTTCTGC9350.012.35281156
CATTCAG13100.011.75502133
AGGGGGG4652.0911102E-811.2985651
CAGTCAC13950.010.78788427
GCTTGAA13450.010.14884261
TGCTTGA13450.010.14834160
CACATTC16250.09.90709831
Produced by FastQC (version 0.11.5)