FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_Al_SPL9_col_B_l2-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_Al_SPL9_col_B_l2-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT120.8784773060029283TruSeq Adapter, Index 7 (97% over 35bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.5124450951683748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.36603221083455345No Hit
GAACAAGCTGAACGAAGTAGCTTCTTCCGTTGTACAACTTTTGGCACTAA20.14641288433382138No Hit
AATACTAGTTTGTCTCATTATTGCCGAGATAATGGCCTACTTCTTCACAT20.14641288433382138No Hit
ATATAGCTTTTGTTTCTATATGAGATGTATCTCCTGCCTTAAACCGATGT20.14641288433382138No Hit
GCTACCATGACGACGTGTACGACGGTTACGCGGAATACGACAACCTTCAA20.14641288433382138No Hit
ATCTTTCTTTAGTAGTATAATAGCAAAGTCGCAATCCATTCATGAATTGT20.14641288433382138No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT20.14641288433382138No Hit
GTAAAGCAAGGATGAGTACACATCAACGTACTCAGCAAATGTCCCGTAGA20.14641288433382138No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA20.14641288433382138TruSeq Adapter, Index 7 (97% over 35bp)
AACAAACAAATATTACTCGATAAATAGACATGTGATATTGAATTAAACGT20.14641288433382138No Hit
GTACTCATCAAGAAATGGGAAGGAGATTATGTCCTTTTTTCTTCTCCTGA20.14641288433382138No Hit
AAAAGTGAGTAAACAGAATTCCATACTCGATTTCAGAGATACATATATAA20.14641288433382138No Hit
GGAAGTCACCCATCCTAGTACTACTCTCGCCCAAGCACCCTTAACTGCGG20.14641288433382138No Hit
AGAAAACGGATTGTCCTTTGGTATAGAATAGTTTCCCCATAGCTGAAACT20.14641288433382138No Hit
TACCACGAGTCATAAAGATCACGCGTCACGAGCATCGTCTTCATCATACT20.14641288433382138No Hit
GTACATTGGTACAAACCATGTTGACACATACTTCTTGGAGTCACAACTGG20.14641288433382138No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC20.14641288433382138TruSeq Adapter, Index 7 (96% over 32bp)
ACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTA20.14641288433382138No Hit
AAGAACTTAAACCGCAACCGGATCTTAAAGGCGTAAGAATTGTATCCTTG20.14641288433382138No Hit
AAGTCGAATATGACTTGATGTCATGTGTATCATTGAGTATAAGAACTTAA20.14641288433382138No Hit
ATCTAGCGACCATTTGGCGACGGTTTGACAGCTACAGTACGACAACACGA20.14641288433382138No Hit
GGTACTACGAGCCCTCTGTCCCACACATCTAACCAGCTCGCGTGGTTCAC20.14641288433382138No Hit
ATACGATTGCATTGCATATACTAACGGATGCGATCATACCAGCACTAATG20.14641288433382138No Hit
CTATTAGATTTTGTTTTTGGGTGACTAACTCTCATTGCTACGAAGTCGAA20.14641288433382138No Hit
ACGACTATATAGAACCAGTGCTTTAGTACCATACTAATTGCAGTGAAGAT20.14641288433382138No Hit
ACCAAAGTCGTACAATTAGGACCTCGATATGGTTCTATTCTGTTCATCGT20.14641288433382138No Hit
AAGTGGATCCATTTGTGATTTGGAGAAAGATGTTGATGAGCAAGAATGTA20.14641288433382138No Hit
TTGTCGAGATCTCATAAGGGAAGGTACCTTCCAATGATATGTCATATGAG20.14641288433382138No Hit
GTAATGTTTTCTAATCAAAAATAGATCAATGTCTGTTTTTAATAATCCCT20.14641288433382138No Hit
ATTTTTGTTTGCTCGGTTTGGATAGATTAGTTCAGCATTTTGTTCGTTTG20.14641288433382138No Hit
AATTAGTCGCCATTATAGTACGACTACTCAACGACTAATTTATATTGTAT20.14641288433382138No Hit
CCATTCATCGTCCTGGTCGAGGGTTCCGCCGTCCTGGTCGACGAATTCTC20.14641288433382138No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)