FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_AL_SPL9_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_AL_SPL9_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length76
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTGATGTGCCGGGAATCAAAAGGTTACTTCCAAAAGAATGGAGGGAA23.571428571428571No Hit
GAGGGTTTTTATACATTAAAATATATTTTCTTTAGTATTTGATAATTTAA11.7857142857142856No Hit
GATTTAATATTTTATAATTTTTTAACTTAAATATGTATATATTTTTATTT11.7857142857142856No Hit
CCACATTTGTTTACGGATGTAAACAATACAAGGACATTCACGAGTGAGAC11.7857142857142856No Hit
GAAATCTCGGATGCGGAAAAGTTGTCGACGGTGACTCGAAGTGATTCAGT11.7857142857142856No Hit
ATAAATAACAAACCACTTAATTCTATTCTTTTTGATTTGTTTAAAAAGAA11.7857142857142856No Hit
TTAAAACTAACAATTTCATTCAAAAAACTATAAAACAAATTAAAGAACAC11.7857142857142856No Hit
ATACAAAAAATAAAAAGATAACATAAAGACCAAACTTTAACAAAACTAAA11.7857142857142856No Hit
CTCCTCCTAGATGGTTTGGCCTAAAAAGACCGTTGTTGCATAAGTTGGAG11.7857142857142856No Hit
AATGTTGTAAAACAACTTAGAATTTCAACAAAAACCGGTTAATTGCAAGC11.7857142857142856No Hit
TAATCAATTTAATTTAGTCACTCTCATGGATCATACATTTTCTCTGTCAA11.7857142857142856No Hit
GAATTGTCCTTTGTATAATGATTTTCTGTTTTTAATTCATTTGATTTTGA11.7857142857142856No Hit
TACAAAAACTAAATTGAAGTACTTAGCACTGATGATGATGATTGATGACT11.7857142857142856No Hit
CGAAAACATCTTACAAATTTCATATTACTGTTCTCTAAAAATAGCAAATA11.7857142857142856No Hit
ATCTAATAAAATTAAATTAATATTATAAAGTAAATAATAAAAAACAAGAA11.7857142857142856No Hit
AATCGAAATTAAAAAATCTTATAATATTTGAATTGGTAAAAAAATATACT11.7857142857142856No Hit
GTTCAAGCAATCCGACACGGCAGGCACTTCCCCTGGCCTCGCTGCTAACT11.7857142857142856No Hit
ATATAACTATCTGATGATGATGATGAATGGTTATAAATAGATGGAGAAGA11.7857142857142856No Hit
TCTTAGCAGTAACTCTCATGAAAAAATAATGTGGTTTTGATATAAATTCA11.7857142857142856No Hit
ACGGAAGGTTTTAGTAAATTTGGAATTTCTGTCAATAAATATTAAATATG11.7857142857142856No Hit
GTGGTGTAATAAGAAAAGTGTATTGTTAAAATAGATTCCTCCCTATTACC11.7857142857142856No Hit
AAATAAAATCGATTAAACTAAAAAATCAATCTTATTACTAAGACATAAAA11.7857142857142856No Hit
CCATAAGGTGCGTTCACAAAAGCCTGAAGATTTGTCTTCTTCTTTGAGAT11.7857142857142856No Hit
AGAAGGAGATAAAGACTAAGAAGAAGAAGAAAAGTGAGAAGGAGAAGAAG11.7857142857142856No Hit
ATAAAAAGTTTTGTCATTTCAAAAATACTCAAAAAATTAGAATTATTTTT11.7857142857142856No Hit
TAAACTTATTAAAATTATTAAAACAAATCTAATTAATATAATTGAATAAA11.7857142857142856No Hit
CTTTTTTTAGGGGGTCTACAACCATTATGTGGCATAGGGGTTACATCTCG11.7857142857142856No Hit
GACAAGTCATGACCTGAAGAAGATTCACAATTCAAACTCCAGTAATTGCA11.7857142857142856No Hit
ATTTATCATTGATTATAAGAAATTAAACATCAACAGAAAAAAAAAAACGA11.7857142857142856No Hit
TGTTGTGATAATAATCCGACTTCCAGGACCAAACCATCGAGTTTCTTTCG11.7857142857142856No Hit
AAAAAAATAAAAACAAAAAACATGTATAAAATAAAAACACAAAAATAAAA11.7857142857142856No Hit
ACGAAAATAAAATAGGGTTTGGTTGTTTTGGCCGGTTGTTGATGTTTGGC11.7857142857142856No Hit
AAGGATAAGATTTGCTTATTCATAAGTTGTAGAAATGAAAAAAAAATTCG11.7857142857142856No Hit
TACTTTTTCAAAAATTCATATAATTTATATTAAATTATAAATCAAAATTT11.7857142857142856No Hit
GAAAAGGACCGAAACATGCCACTGAAAGACACTACTGAGACAAAGATGGG11.7857142857142856No Hit
AAAACAAAATAAAACGATTCTACCACGATTAATAATTGCTCAAACCACAA11.7857142857142856No Hit
ATTTGTGCAATCTCACGTTCCCGCTCATGTCGAGTTAATGTGGCTTTTAT11.7857142857142856No Hit
AAAAAAAAACAATAATACAAAAAAAAAAAATTAATAAAAAAAATAAAAAA11.7857142857142856No Hit
AAAATCAAGTTCAAATACATAAAATTATCTTGTTTCTAGACAAACAACTT11.7857142857142856No Hit
AAAGCAAAAAACTCTATTTGAAAGCGATTATTACTATAGCTATGAATACC11.7857142857142856No Hit
AAATAACAATACGAACAAATATAATTACAACCTCAACATAATAGAAGCAA11.7857142857142856No Hit
AATCCAATCAAAACTATACGATAAAAAGGGTAAAATTTCAATAATCAAAA11.7857142857142856No Hit
ATAAAAAAAGATTTATAACTTATTATTTTATTCATAATCGAAAACATAGT11.7857142857142856No Hit
GGTACTTACAAGGCCAATGAGAAATCGAACAGCTACTTTGCCAGATATTA11.7857142857142856No Hit
ATTACTAAATCTACAAGTATAAAATATAGCACTATCATTAGAACAAAACA11.7857142857142856No Hit
CAGAAAAAAGTACATAAATGTAGTGAGTGTGGAATTAGAGATTACAGGAA11.7857142857142856No Hit
AAAGTAACCTCTAGAAAAAAAAAAAATTGAAGATAGAATAATATTTACTC11.7857142857142856No Hit
TTAAATTTAGTTTTTACTATAAATGTAAAAATAATTGTATTAATAAAAAC11.7857142857142856No Hit
GACCTCTATATTATATAAGTATGTTAATATATATTCTTACAGTTCTAAGA11.7857142857142856No Hit
AGATCAAAGACAAAATGATTACCTAACCTGCCATGAGTAAAAAAGGATTT11.7857142857142856No Hit
AATTATTAACCTCTAATTGTCGAAACAGAGGAAGCCAGTATTTACTCAAA11.7857142857142856No Hit
TGGTATTAATTTTGTATACGTTGTGAATATGTAGGCATCATGATCTTAGG11.7857142857142856No Hit
ATAAAAAAAAAAAAATGCAAACTCGATTAAAAAGATCAACTACATGGAAG11.7857142857142856No Hit
ATTTACCAATATTAATTTAAAATAACCAAATAATAAAAAATAAGAAAAAC11.7857142857142856No Hit
AATACCTAAAGCAGTAAACAAAATAACTACTACCGGCCAAGCCGCTAAGA11.7857142857142856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATA50.070.049
ACAAAAT50.070.063
TAAATTT50.070.02
AACAAAT50.070.048
TAAATGT50.070.020
CTATAAA50.070.017
TAAATAA50.070.069
AAATTTA50.070.03
TTGTATT50.070.035
TTACTAT50.070.014
AAACACA50.070.059
AATTTAG50.070.04
AAACAAA50.070.047
TAAAAAT50.070.026
TAAAAAC50.070.044
TAAAAAA50.070.054
CACAAAA50.070.062
TATAAAT50.070.018
AAATAAT50.070.029
TTTTTAC50.070.011
Produced by FastQC (version 0.11.5)