FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_AGL18_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_AGL18_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8040561
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13450.025.50090448
CGTATGC13800.025.36136846
GCCGTCT13700.025.03555951
GTATGCC15400.022.72642347
TGCCGTC15200.022.56494350
ATGCCGT16200.021.17204749
CTCGTAT22450.016.36909744
CGCTCAT22400.015.4681734
TCGTATG24600.014.65392145
CCGCTCA25400.013.6412233
CCGTCTT27200.013.2531852
ACCGCTC27450.013.00497732
TCTCGTA30350.012.80017943
TATCTCG29100.012.74866341
CTGCTTG29850.011.49042859
CACCGCT30550.011.456231
ATCTCGT34600.010.72214242
TCACCGC33900.010.53057930
GTCACCG35300.010.21208129
GCTCATT38250.09.97348235
Produced by FastQC (version 0.11.5)