FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_AGL104_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_AGL104_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3333789
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6900.031.4477650
TATGCCG7100.031.05483848
GCCGTCT7450.028.65633851
ATGCCGT8050.027.38998249
CGTATGC8150.026.62448546
GTATGCC8750.024.39882547
TCGTATG11350.018.80966645
CTCGTAT11250.018.04357544
CCGTCTT12100.017.9330252
GATCTCG12450.016.02332341
CTGCTTG12950.015.94517759
CGTCTTC14850.014.61209153
CGATCTC13750.014.5083940
TCTCGTA13950.014.30038643
AGGGGGG4701.0913936E-1113.4124591
ATCTCGT16800.012.0827542
TTACTCG18250.011.88983735
CTTCTGC18000.011.860538556
CTCGATC18350.011.4435938
CGTCCTA3055.1752443E-410.32768264
Produced by FastQC (version 0.11.5)