FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_AGL104_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_AGL104_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9973440
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG40300.039.1670442
CGGGGGT25500.031.841644
AATCGGG54100.030.1465741
TCGGGGG41600.028.94090843
CGGGGTG10500.026.33245853
GAATCGG66650.024.4175540
AGAATCG82350.020.44234539
TCCGGAG89400.020.16132234
AGGGGGG21550.019.5027961
ACTCCGG93750.019.3378332
GCGGGGT15350.019.15245652
CCGGAGA94650.018.96906935
CTCCGGA96450.018.72391733
TCGGGGT18750.018.47910743
CGGAGAA99900.018.25246436
CACTCCG100950.017.95851731
TGCGGGG9600.016.77027551
GGAGAAT108300.016.70749537
CGGGGTT20100.016.36736944
CGGGGGG24000.016.33254444
Produced by FastQC (version 0.11.5)