FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_nlp7_pchx_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_nlp7_pchx_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6490375
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC125210.19291643395027253No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGC117750.18142249099628296No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA90440.13934479902933192No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGT68110.10494000731852936No Hit
CGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGT66200.10199718814398243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT8000.014.2276461
ACTCTAG28800.013.2921775
GACTCTA30700.013.0312244
CGGGAAT37500.012.5088311
CGTAGCG7750.012.46145625
TAAGCGG11250.012.26261620
CGGGTGT8050.011.9969611
TCTAGCA52700.011.9103097
GACTGTC13800.011.7458474
CGCGAAT7050.011.7417071
CCTGGGG62500.011.64335310
CTAGCAG26100.011.6280748
GTGTGCG8650.011.56238658
GGAGTAC84500.011.5096293
CGCTATG11450.011.44679429
GCTGACT28050.011.3126431
ATAGACG48200.011.31131951
CCCGACT16200.011.2841791
GGGGCAC63850.011.23512913
AGGGCGG51550.011.1726659
Produced by FastQC (version 0.11.2)