FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_nlp7_pchx_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_nlp7_pchx_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3323322
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC46820.14088312838779993No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGC44280.13324017353720163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG5600.017.2447878
GTGCGAG7250.016.17466410
GTGCGCA3251.2369128E-1015.92832968
CGGGGAT7150.015.9201811
TGCGCAT3454.8912625E-914.00356569
TGCGAGT7900.013.97067111
GCTGACT12150.013.911051
CGGCAGT4052.3283064E-1013.62715151
GCCCGTA5900.013.45379367
TGTGCGA7450.013.42567359
CGGGACT4457.4578566E-1113.1773781
CTGACAT10250.013.1244411
TCTAGCA23000.013.0460567
CCCGTAT6350.013.04367268
TATTCGT8200.013.03952618
CTAGCAG12700.013.035438
CTACCCG6650.012.9662019
GACTCTA15300.012.8494434
GTATTCG7800.012.82343217
ACTCTAG15450.012.7246915
Produced by FastQC (version 0.11.2)