FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_nlp7_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_nlp7_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19192607
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC421260.21949076537648066No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGC380440.19822215918869177No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA287270.14967742527109526No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC230460.12007748608617891No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGT203100.10582199698039979No Hit
CGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGT202200.1053530664177097No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTG201440.10495708060921584No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCG195440.10183087685794849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG77350.014.0906095
GACTCTA80400.013.6419114
GCTGACT67450.013.6039461
TCTAGCA144250.013.4371297
CTAGCAG71150.013.37896258
CGGGAAT112550.013.301771
CTGGCCC155600.012.70997166
GGAGTAC268150.012.5670173
GCGGGTC137250.012.2135422
CGCGGGT143950.012.101661
GTACAAC285300.012.0893346
GGCCCAC151900.012.08756168
CCTGGGG188750.012.06006910
CCTGGCC166050.011.99320765
CCCTGGC166500.011.96082364
CGCGGGG20600.011.8893011
CGGGTCT144700.011.8468613
CTGGGGC200450.011.68308811
CGGCAAT35200.011.6619051
GGGGCAC191950.011.66151613
Produced by FastQC (version 0.11.2)