FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_nlp7_mchx_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_nlp7_mchx_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5864530
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGC171450.29235079366973993No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC168240.2868772092563257No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA113300.19319536262923032No Hit
CGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGT105920.1806112339778294No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC97980.16707221209542794No Hit
CTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAA95600.16301391586367536No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTG94410.16098476774779905No Hit
CGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACT91030.1552213050321168No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGT88030.15010580558032785No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCG87000.14834948410188029No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTG80510.1372829536211768No Hit
GTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGC72800.12413612003007915No Hit
CTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAG64980.11080171812574921No Hit
CTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTC64850.11058004648283835No Hit
CGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGA58830.1003149442495818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT25750.015.67398551
CTGACTC25500.015.0139282
ACTCTAG28750.014.8761895
CTAGCAG25950.014.6205318
GACTCTA30550.014.451294
CGCGAAT4850.014.2252231
TAAGCGG9500.014.16001320
GGAGTAC99000.014.1101423
CGCGGGT68600.014.0297811
TGGAGTA101250.013.7965832
GTGTGCG7750.013.7964668
GTACAAC101100.013.680476
TACAACT103750.013.5968837
GCGGGTC67850.013.4204052
CCTGGGG69100.013.37729410
CGGGTCT69550.013.3403343
GAGTACA105750.013.3398444
CCGGCAT8800.013.3280621
GTGCGAG9400.013.20949510
ACAACTA104950.013.1786168
Produced by FastQC (version 0.11.2)