FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_hap2c_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_hap2c_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46880822
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT3784150.8071850787940535No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGT2102060.4483837762059718No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT2083220.444365075339336No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA1993870.4253061091804235No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGTCC1581800.33740875959896777No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGG1445930.3084267592406976No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT1349860.2879343711166157No Hit
GGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTC1235420.26352353634072373No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC1119000.2386903540215229No Hit
CGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT1101960.23505560546698603No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTC1082910.2309921101639387No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGG1055790.2252072286616476No Hit
CGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCT1038220.2214594274818816No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTC924410.19718297601522433No Hit
CGGAGGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCA896530.19123598131449146No Hit
GGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCA882790.18830514533213602No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGC878210.18732820000468423No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTG877430.18716182066944134No Hit
GTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTG867560.18505648215809867No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTC831260.1773134438641029No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAGCTTGATGTCG823520.17566244892207736No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCATGGAGGG820870.17509718579593164No Hit
GGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGT807730.1722943339176092No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGGATGTCGGCGGG772740.16483072758408543No Hit
TGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGA736490.15709835463209243No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA726320.15492902406873327No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC709720.15138813052382058No Hit
CTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGAC696770.1486258069451086No Hit
CCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGG686510.14643727876614449No Hit
GTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTC684570.14602346349643783No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACC656910.1401233963005171No Hit
CGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCA652230.13912512028905977No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACG645210.13762770627187382No Hit
CGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGGATGTCGGCGGGGTGCTTCACGTAGG632330.13488031417196567No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA629500.13427665581461007No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCG612780.1307101654488908No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG612150.13057578213965618No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGT601290.12825926985665909No Hit
CGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGT600360.12806089449540795No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTC582340.1242171052376172No Hit
GTCGGAGGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGT564100.12032638847501435No Hit
GCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGA563250.12014507766096763No Hit
GTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCC521730.11128857766188485No Hit
CCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGC508160.10839400384233877No Hit
CACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACC503020.10729760668445618No Hit
CGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTC499220.10648704069224724No Hit
GGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACT495050.10559755116921797No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGATGAGGCTGAAGCTGA487130.10390816099598253No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTT483410.10311465955097801No Hit
CGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAGCACCC483210.10307199818296701No Hit
CTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGCC480620.10251953346722462No Hit
CCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACG478390.10204385921390201No Hit
CTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT477810.10192014124667012No Hit
ACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA469940.10024141641543742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT1081950.026.51795669
GTGCGCA1088600.026.3458768
GGTGCGC1120400.025.65731667
AGGTGCG1143700.025.1105866
AAGGTGC1259600.022.93186265
TCAAGGT1307850.022.10453263
GCTGACT156450.021.8283671
TTCAAGG1437750.020.09273762
CGCTTCA1489300.019.29744159
GACTCTA210300.019.2217524
ACTCTAG201250.019.1434045
CTAGCAG187050.018.69718
GCGCTTC1538700.018.62852958
CTGACTC189200.018.3031582
TGCGCTT1581450.018.13476857
CCGGGGT48850.017.1594491
GTCGGGA936700.017.0875231
TCGGGAT994450.016.124642
ATGCGCT1784300.016.07318556
CGACGAT1898750.015.8874311
Produced by FastQC (version 0.11.2)