FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_cdf1_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_cdf1_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13329610
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG15200.013.1639534
CGCGGGG15200.012.7089711
GTGTGCG20500.011.9451238
CCGGGAT33050.011.8986941
TACCTAG22700.011.2433379
ACCTAGG21500.011.22945610
ATAGGGG15850.011.10542161
CGGCAAT33900.010.8880481
CGGGGAT25700.010.872211
GTGCGAG26350.010.86417210
CCCGGCT22100.010.7701751
CGCGAAT17400.010.7055861
TATAGGG15600.010.398108560
CGGATAT39150.010.3971951
GTCGAAT33850.010.1907771
CGGCAAC72700.010.0592921
CCGGCAT25400.010.0499511
TAAGCGG26050.09.93011520
TGCGAGT30350.09.88688911
CGTAGCG22350.09.87735525
Produced by FastQC (version 0.11.2)