FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_cdf1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_cdf1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781402
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA6750.016.86331
GCGGAAT4950.013.9366111
GTTAGTT6700.013.900211
TGCGAGT8350.013.63078511
CCGGGAT6400.013.4738721
CTATGCG4901.8189894E-1213.38113654
GTGCGAG8150.013.11913710
CGCTATG9050.012.95920629
GACTGTC8850.012.86068254
GTCGCTA9450.012.77568727
ATTAGGG6750.012.7740796
GCGACTG5155.456968E-1212.7244727
TATGCGG5205.456968E-1212.60937455
TAGCCCG4408.62201E-1012.54588234
ACCTAGG5257.2759576E-1212.48232510
TATAGGG4154.827598E-912.47434460
CGTGAAC6650.012.45648164
CCGGTAT7500.012.41752051
GTGTGCG6050.011.971748
CTGACAT10100.011.9530591
Produced by FastQC (version 0.11.2)