FastQCFastQC Report
Fri 11 May 2018
HW73GBGX5_n01_cdf1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW73GBGX5_n01_cdf1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9640306
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAG20950.012.18284110
GCCAGTA16250.012.0996881
CCGGCAT20950.011.5256831
CCGGGAT25450.011.3852921
TGCGAGT23150.011.02507611
ACCTAGG15700.010.9842710
CTGACAT33000.010.9755941
GACTGTC28850.010.879134
CGGCAAT23600.010.8161421
TACCTAG15450.010.4920719
GTCGAAT28350.010.3423341
GTGTGCG15750.010.2920638
GCCCGTA13000.010.08830667
CCCGTGA18500.010.07432362
TAAGCGG23450.09.70722720
CGGACAT22050.09.6991981
TAGCCCG12450.09.6985234
CGGCAAC58200.09.6608931
ACATGCG12550.09.6188454
CGGGTGT10400.09.6187211
Produced by FastQC (version 0.11.2)