FastQCFastQC Report
Wed 9 Oct 2024
HW737BGXW_n01_SRS_S2_WT_clone1_Rep2_ox_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW737BGXW_n01_SRS_S2_WT_clone1_Rep2_ox_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946320
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC465621.5803442938988297RNA PCR Primer, Index 4 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC112830.3829522930299492RNA PCR Primer, Index 4 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC83260.28258980694561353RNA PCR Primer, Index 4 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC74200.25183958293735914RNA PCR Primer, Index 4 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC68130.23123761166472073RNA PCR Primer, Index 4 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC67970.23069456135110916RNA PCR Primer, Index 4 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC48820.16569822694072606RNA PCR Primer, Index 4 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC39940.1355589345352847RNA PCR Primer, Index 4 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG36690.12452822504004997RNA PCR Primer, Index 4 (100% over 49bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC36470.12378153085883406RNA PCR Primer, Index 4 (100% over 50bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC36420.12361182763583045RNA PCR Primer, Index 4 (100% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC36350.12337424312362541RNA PCR Primer, Index 4 (100% over 44bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC34170.11597518260066796RNA PCR Primer, Index 4 (100% over 44bp)
AACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC33980.11533031035325422RNA PCR Primer, Index 4 (100% over 44bp)
CACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC33120.11241141491759213RNA PCR Primer, Index 4 (100% over 44bp)
TATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC32830.11142713622417116RNA PCR Primer, Index 4 (100% over 44bp)
TTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC31560.10711667435987944RNA PCR Primer, Index 4 (100% over 44bp)
ACCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC30150.10233104347117761RNA PCR Primer, Index 4 (100% over 44bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC29840.10127888348855521RNA PCR Primer, Index 4 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)