FastQCFastQC Report
Wed 9 Oct 2024
HW737BGXW_n01_SRS_S2_Hen1_clone3_Rep2_ox_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW737BGXW_n01_SRS_S2_Hen1_clone3_Rep2_ox_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161558
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC587371.8578498322662433RNA PCR Primer, Index 3 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC146320.46280979188109156RNA PCR Primer, Index 3 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC111810.3536547487030129RNA PCR Primer, Index 3 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC97670.3089299642771064RNA PCR Primer, Index 3 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC90640.28669409196351925RNA PCR Primer, Index 3 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC89440.28289849498253705RNA PCR Primer, Index 3 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC60580.1916143875899161RNA PCR Primer, Index 3 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC52170.16501357874819947RNA PCR Primer, Index 3 (100% over 44bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC51340.16238829083635348RNA PCR Primer, Index 3 (100% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC50170.15868758377989586RNA PCR Primer, Index 3 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG48070.15204528906317707RNA PCR Primer, Index 3 (100% over 49bp)
CACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC44230.13989937872403416RNA PCR Primer, Index 3 (100% over 44bp)
AACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC44200.1398044887995096RNA PCR Primer, Index 3 (100% over 44bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC43570.13781180038449398RNA PCR Primer, Index 3 (100% over 50bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC43270.13686290113924843RNA PCR Primer, Index 3 (100% over 44bp)
TATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC43170.13654660139083324RNA PCR Primer, Index 3 (100% over 44bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC42910.13572422204495377RNA PCR Primer, Index 3 (100% over 44bp)
TTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC42570.13464880290034217RNA PCR Primer, Index 3 (100% over 44bp)
AATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTAT40590.1283860678817216RNA PCR Primer, Index 3 (100% over 48bp)
TGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC40410.1278167283345743RNA PCR Primer, Index 3 (100% over 44bp)
CTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC39560.12512818047304525RNA PCR Primer, Index 3 (100% over 44bp)
ACCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC39140.1237997215297015RNA PCR Primer, Index 3 (100% over 44bp)
CAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC38440.12158562329079524RNA PCR Primer, Index 3 (100% over 44bp)
TTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC38280.12107954369333093RNA PCR Primer, Index 3 (100% over 44bp)
CTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC37350.11813795603306976RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAGCTCGTATGC36460.11532288827217467RNA PCR Primer, Index 3 (98% over 50bp)
ATCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC34100.10785821420957642RNA PCR Primer, Index 3 (100% over 44bp)
CCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC33200.1050115164738398RNA PCR Primer, Index 3 (100% over 44bp)
CATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC32440.10260763838588444RNA PCR Primer, Index 3 (100% over 44bp)
CCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC31740.10039354014697817RNA PCR Primer, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)