FastQCFastQC Report
Wed 9 Oct 2024
HW737BGXW_n01_SRS_S2_Hen1_clone2_Rep2_ox_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW737BGXW_n01_SRS_S2_Hen1_clone2_Rep2_ox_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556378
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC641372.508901265775249RNA PCR Primer, Index 2 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC93840.36708186348028343RNA PCR Primer, Index 2 (100% over 44bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC68060.266236057421868RNA PCR Primer, Index 2 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC64350.2517233366896445RNA PCR Primer, Index 2 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC63800.24957185517947658RNA PCR Primer, Index 2 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC58420.22852645422547055RNA PCR Primer, Index 2 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC56490.22097671001706318RNA PCR Primer, Index 2 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC55020.21522638670806898RNA PCR Primer, Index 2 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC51930.20313897240548934RNA PCR Primer, Index 2 (100% over 44bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC49800.19480687128429364RNA PCR Primer, Index 2 (100% over 44bp)
CTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC49120.19214685778081333RNA PCR Primer, Index 2 (100% over 44bp)
TTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC48930.1914036187136644RNA PCR Primer, Index 2 (100% over 44bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC47520.1858880024785067RNA PCR Primer, Index 2 (100% over 44bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC45870.179433557948003RNA PCR Primer, Index 2 (100% over 50bp)
TGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC43330.16949762515559125RNA PCR Primer, Index 2 (100% over 44bp)
ATGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC37650.14727868883240272RNA PCR Primer, Index 2 (100% over 44bp)
TTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC35990.1407851264562596RNA PCR Primer, Index 2 (100% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC35060.13714716681179387RNA PCR Primer, Index 2 (100% over 44bp)
CCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC34650.13554333514057781RNA PCR Primer, Index 2 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG31490.12318209591852221RNA PCR Primer, Index 2 (100% over 49bp)
AACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC31310.12247797469701273RNA PCR Primer, Index 2 (100% over 44bp)
CACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC31300.12243885685137332RNA PCR Primer, Index 2 (100% over 44bp)
TATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC29790.11653206215982144RNA PCR Primer, Index 2 (100% over 44bp)
ACCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC29180.11414587357581703RNA PCR Primer, Index 2 (100% over 44bp)
CTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC27930.10925614287088999RNA PCR Primer, Index 2 (100% over 44bp)
CCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC27110.10604847952845785RNA PCR Primer, Index 2 (100% over 44bp)
AGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC26110.10213669496451622RNA PCR Primer, Index 2 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAGCTCGTATGC25990.1016672808168432RNA PCR Primer, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)