FastQCFastQC Report
Wed 9 Oct 2024
HW737BGXW_n01_SRS_S2_Hen1_clone1_Rep2_ox_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW737BGXW_n01_SRS_S2_Hen1_clone1_Rep2_ox_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010727
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC485881.6138294837094163RNA PCR Primer, Index 1 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC105550.35057977691102515RNA PCR Primer, Index 1 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC78640.2611993714474942RNA PCR Primer, Index 1 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC69940.23230269632550543RNA PCR Primer, Index 1 (100% over 44bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC65410.21725649652060783RNA PCR Primer, Index 1 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC63830.21200859460190183RNA PCR Primer, Index 1 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC63050.20941785821165454RNA PCR Primer, Index 1 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC62180.20652819069945563RNA PCR Primer, Index 1 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC53520.17776437385388977RNA PCR Primer, Index 1 (100% over 44bp)
TTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC48920.16248567206525202RNA PCR Primer, Index 1 (100% over 44bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC47040.15624133307337398RNA PCR Primer, Index 1 (100% over 44bp)
TGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC45010.14949877554490992RNA PCR Primer, Index 1 (100% over 44bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC43590.14478230673189565RNA PCR Primer, Index 1 (100% over 44bp)
CTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC42990.1427894325855516RNA PCR Primer, Index 1 (100% over 44bp)
AACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC38470.1277764473497597RNA PCR Primer, Index 1 (100% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC38340.12734465795138517RNA PCR Primer, Index 1 (100% over 44bp)
ATGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC36090.11987137990259494RNA PCR Primer, Index 1 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG34350.1140920448781972RNA PCR Primer, Index 1 (100% over 49bp)
TTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC33740.11206595616274741RNA PCR Primer, Index 1 (100% over 44bp)
CACATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC33230.11037201313835496RNA PCR Primer, Index 1 (100% over 44bp)
ACCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC31440.10442660526842852RNA PCR Primer, Index 1 (100% over 44bp)
TATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC30900.10263301853671888RNA PCR Primer, Index 1 (100% over 44bp)
CCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC30640.10176943973996977RNA PCR Primer, Index 1 (100% over 44bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC30520.10137086491070098RNA PCR Primer, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)