FastQCFastQC Report
Wed 9 Oct 2024
HW737BGXW_n01_SRS_FVB_4N_Rep3_unox_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW737BGXW_n01_SRS_FVB_4N_Rep3_unox_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6688723
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC430730.6439644757302702RNA PCR Primer, Index 24 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTC76170.11387823953839919RNA PCR Primer, Index 24 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)