FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27091096
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378669213.977625711414554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA52450.023.54705262
CCGTATC50300.023.09267261
CGTCTAG23300.022.3930741
GCCGTAT51600.022.1721960
CGCCGTA53550.021.70096259
TCTCGGT57250.021.11259849
GTAGATC68950.019.88819144
GATCTCG65650.019.79411947
TAGACTG64300.018.6788655
CTAGACT62450.017.5917134
TCGCCGT64650.017.32614958
TAGATCT88150.016.80746345
CTCGGTG69150.016.46610550
TCTAGAC69050.016.255373
CTAAGTG59850.015.95517938
GTATCAT82600.015.71352863
AAGTGTA65750.015.6405640
TCGGTGG74800.015.03347851
TGTAGAT91450.014.95508743
TCTAAGT69750.014.8410137
Produced by FastQC (version 0.11.5)