FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54472138
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1114690.20463489059305878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG64650.016.6271532
CTAGACT19350.015.73502354
TAGATCT78800.014.78994945
CGTCTAG12550.014.5195931
TCTAGAC20900.013.5653573
AGGTGGG296850.013.49925970
TAAGGTG478150.013.14569970
CCGACGA1431550.012.85940170
GCTCGGA92850.012.190331
GTGTAAG118250.011.8833271
TCTAGGG12450.011.8078953
ATAAGGT538200.011.75687469
GCCGACG1822450.011.56257370
CTATACT72650.011.5130774
AAGGTGG451750.011.29538170
TCTAGGA22850.010.8797832
TATACCG193300.010.8446665
GGTGGGT297050.010.591845570
TACACTG134150.010.5653915
GTTCTAG10650.010.5291961
Produced by FastQC (version 0.11.5)