FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49156681
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2113870.4300269987715403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC16150.015.3881573
CTCGGAG55600.014.6103832
CGTCTAG10700.014.4106451
CTAGACT15650.013.4177544
CCGACGA1044450.012.3310670
CTATACT63500.011.7945934
TACACTG119500.011.77292255
CGACGAG784700.011.68078770
TCTATAC70200.011.1190733
GTGTAAG105550.011.0560641
TATACCG173700.011.0006665
AGGATAC373100.010.89979969
CCCTAGA13900.010.8409711
GACGAGG602500.010.76362770
GCCGACG1325350.010.76326170
GCTCGGA80250.010.6551441
TACACCG269800.010.59756855
CTTACAC117600.010.4174533
CTACACT93250.010.3961814
CCTATAC57350.010.3146413
Produced by FastQC (version 0.11.5)