FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64294276
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTCTAAGTGTAGATC3847250.5983814173442128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1735630.2699509362233117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT562850.055.01122745
GTAGATC602700.050.8151644
GTATCAT610700.049.83384363
AGATCTC630550.049.1880846
AGTGTAG621950.049.06824541
CGTATCA627300.048.30299462
AAGTGTA643200.047.83893640
CTAAGTG648700.047.1262938
TGTAGAT649800.046.9918543
TAAGTGT656450.046.6601639
GTGTAGA653900.046.52601642
ATCTCGG661550.045.9309548
TCTCGGT660050.045.74907349
CCGTATC660800.045.29265261
GATCTCG695550.044.1134847
GCCGTAT686550.043.57353260
CTCGGTG689650.043.42519850
TATCATT736300.041.43306464
TCTAAGT746000.041.4157737
TCGGTGG743200.040.26788751
Produced by FastQC (version 0.11.5)