FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950488
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACAGAACCTGTGTAGATC270082.8414877410340793No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA54710.5755990606930335No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC17790.1871670131553476No Hit
GCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATC12540.13193222849736136No Hit
GATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGAACGTATGGGC12520.13172181027009283No Hit
CCGAGCCCACGAGACACCGCTGTCTCTTATACACATCTGACGCTGCCGAC12310.12951241888377338No Hit
CCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATT12220.12856553686106506No Hit
GGTCTAATCAGCCCATGACCAACATAACTGTGGTGTCATGCATTTGGTAT11960.12583009990657432No Hit
CCATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCG11880.12498842699750023No Hit
ATCTTAAACACATTAAACTAATGTTATAAGGACATATCTGTGTTATCTGA11870.12488321788386597No Hit
GACATACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCT11120.11699253436129652No Hit
ACATAACTGTGGTGTCATGCATTTGGTATCTTTTTATTTTGGCCTACTTT11110.11688732524766225No Hit
GTGTATGTCAGATAACACAGATATGTCCTTATAACATTAGTTTAATGTGT10550.11099561488414372No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC10370.10910185083872705No Hit
CAGTTATGTTGGTCATGGGCTGATTAGACCCGATACCATCGAGATGTCTT10060.10584036831606501No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA9760.10268409490703723No Hit
GTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTA9580.10079033086162056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA301.0233998E-770.0068411
GCTTGCG207.924074E-570.0068411
CACTCCG207.924074E-570.0068431
GGACCGT150.00222392370.0068424
TCCGGAC301.0245276E-769.995817
GCGTCCC150.002225315769.995847
GTACCGA252.8416289E-669.9957946
GTTCGGG353.7143764E-969.9957950
GCTAGCG252.8416289E-669.9957947
ACGCGCT353.7143764E-969.9957957
CGAGTCT150.002226244769.9884343
CATGCGA252.843406E-669.988434
ACGTTAC150.002226244769.9884366
TCCGACT150.002226244769.9884338
TACATCG207.934406E-569.9884366
AACGCGT150.002226244769.9884341
AGTCGGA252.843406E-669.9884367
CGCCGTA30300.066.9926759
CGTATCA30500.066.7758462
CCGTATC30300.066.5236661
Produced by FastQC (version 0.11.5)