FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438153
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGGGTGTAGATC6320314.424869851398942No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA37580.8576912630975937No Hit
GTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTA17400.3971215534299663No Hit
CCGAGCCCACGAGACACCGCTGTCTCTTATACACATCTGACGCTGCCGAC16020.36562570608896894No Hit
GTCATGGGCTGATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGA14700.33549924341497145No Hit
GACATACACCATACAGTCATAAACTCTTCTCTTCCATATGACTATCCCCT13810.31518670418780653No Hit
AAATAAGACATCTCGATGGTATCGGGTCTAATCAGCCCATGACCAACATA13230.30194931907347433No Hit
CCATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCG13080.2985258574059746No Hit
GCCCATGACCAACATAACTGTGGTGTCATGCATTTGGTATCTTTTTATTT9410.21476516194114842No Hit
GTCTTATTTAAGGGGAACGTATGGGCGATAACGCATTTGATGGCCCTGAA9160.20905939249531558No Hit
CCATGAATATTATCTTAAACACATTAAACTAATGTTATAAGGACATATCT9160.20905939249531558No Hit
ACGTATGGGCGATAACGCATTTGATGGCCCTGAAGTAAGAACCAGATGTC8400.1917138533799837No Hit
TGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCGTGAAA8220.18760569937898405No Hit
GCCCTGAAGTAAGAACCAGATGTCTGATAAAGTTTCAGTTTAGCTACCCC8170.18646454548981747No Hit
GTCCTTTCATGCCTTGACGGCTATGTTGATGAAAGTAGGCCAAAATAAAA7830.17870469904348482No Hit
CGTATGGGCGATAACGCATTTGATGGCCCTGAAGTAAGAACCAGATGTCT7730.17642239126515166No Hit
ACACCACAGTTATGTTGGTCATGGGCTGATTAGACCCGATACCATCGAGA7670.1750530065981518No Hit
GGTCTAATCAGCCCATGACCAACATAACTGTGGTGTCATGCATTTGGTAT7420.16934723715231892No Hit
CTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACC7230.16501085237348598No Hit
GTATCGGGTCTAATCAGCCCATGACCAACATAACTGTGGTGTCATGCATT7050.16090269837248633No Hit
CCGTGAAACCAACAACCCGCCCACCAATGCCCCTCTTCTCGCTCCGGGCC6890.1572510059271533No Hit
GCATTGGTGGGCGGGTTGTTGGTTTCACGGAGGATGGTAGATTAATAGAC6680.1524581595926537No Hit
ATCTTAAACACATTAAACTAATGTTATAAGGACATATCTGTGTTATCTGA6250.1426442361458212No Hit
CTTGACGGCTATGTTGATGAAAGTAGGCCAAAATAAAAAGATACCAAATG6240.1424160053679879No Hit
CCGTAGGTGCGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTAT6230.14218777459015458No Hit
AGCTAGTACATTAAATCAATGGTTCAGGTCATAAAATAATCATCAACATA6200.14150308225665464No Hit
CATTAAATCAATGGTTCAGGTCATAAAATAATCATCAACATAAATCAATA6190.14127485147882132No Hit
GGACATATCTGTGTTATCTGACATACACCATACAGTCATAAACTCTTCTC6150.14036192836748806No Hit
GCATATAAGCTAGTACATTAAATCAATGGTTCAGGTCATAAAATAATCAT6030.13762315903348832No Hit
CACCAATGCCCCTCTTCTCGCTCCGGGCCCATTAAACTTGGGGGTAGCTA5880.1341996973659886No Hit
AAAGGACAGCACACAGTCTAGACGCACCTACGGTGAAGAATCATTAGTCC5800.13237385114332206No Hit
CTTTATCAGACATCTGGTTCTTACTTCAGGGCCATCAAATGCGTTATCGC5750.1312326972541555No Hit
ACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCCGTGAAACC5490.12529869703048935No Hit
ATATTATCTTAAACACATTAAACTAATGTTATAAGGACATATCTGTGTTA5390.12301638925215622No Hit
ACCATCCTCCGTGAAACCAACAACCCGCCCACCAATGCCCCTCTTCTCGC5370.12255992769648959No Hit
GAGGATGGTAGATTAATAGACCAAATGGGGAAGGGGATAGTCATATGGAA5310.1211905430294897No Hit
ACATAAATCAATATATATACCATGAATATTATCTTAAACACATTAAACTA5220.11913646602898988No Hit
GTCATATGGAAGAGAAGAGTTTATGACTGTATGGTGTATGTCAGATAACA5180.11822354291765663No Hit
CTAATGATTCTTCACCGTAGGTGCGTCTAGACTGTGTGCTGTCCTTTCAT5160.11776708136199No Hit
GGCATTAAGAGGAGGGGGTGGGGGGTTTGGAGAGTTAAAATTTGGTATTG5080.11594123513932347No Hit
CTACCATCCTCCGTGAAACCAACAACCCGCCCACCAATGCCCCTCTTCTC5040.11502831202799021No Hit
GTCATGCATTTGGTATCTTTTTATTTTGGCCTACTTTCATCAACATAGCC4960.1132024658053237No Hit
ATGCGTTATCGCCCATACGTTCCCCTTAAATAAGACATCTCGATGGTATC4950.11297423502749039No Hit
GCGTTAACCCTGCGCGATTAAAGGCAGTATCCAGTTCTGAACGTCCGGTA4790.10932254258215737No Hit
CTTCAGGGCCATCAAATGCGTTATCGCCCATACGTTCCCCTTAAATAAGA4760.10863785024865744No Hit
GATTAGACCCGATACCATCGAGATGTCTTATTTAAGGGGAACGTATGGGC4700.10726846558165755No Hit
GGATAGTCATATGGAAGAGAAGAGTTTATGACTGTATGGTGTATGTCAGA4670.10658377324815761No Hit
AATTTACATAGTACAACAGTACATTTATGTATATCGTACATTAAACTATT4650.10612731169249098No Hit
AAGAGGGGCATTGGTGGGCGGGTTGTTGGTTTCACGGAGGATGGTAGATT4570.10430146546982447No Hit
TTCTTCTACTATCCCCAATCCTAATTTCAATATCAAACCTAATTAAACAC4480.10224738846932464No Hit
GCCCGGAGCGAGAAGAGGGGCATTGGTGGGCGGGTTGTTGGTTTCACGGA4450.1015626961358247No Hit
GGTTTGGAGAGTTAAAATTTGGTATTGAGTAGCATTTATGTCTAACAAGC4390.10019331146882482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA500.070.126811
CCGTAGT150.00220807570.12681
AACGACT353.6543497E-970.12681
AGTTAGT301.0100666E-770.12681
ATTTAGG252.8364357E-670.0067413
ACCGGTC150.002223134570.0067415
GCGACCC401.3460522E-1070.0067412
GCGAATG252.8364357E-670.0067416
GAGTCGA150.002223134570.0067411
TTGCTAG500.070.0067417
GAGTATC150.002223134570.0067412
TAGCGTC401.3460522E-1070.0067416
TTAGAGC150.002223134570.006743
CATTGCG252.8364357E-670.0067412
CGTGACG150.002223134570.0067417
CCGTGAT301.0221265E-770.0067415
GCGCCTA207.9193735E-570.0067416
ATTCGGC150.002223134570.0067411
TGACGTA207.9193735E-570.0067413
GCGCATA301.0221265E-770.0067411
Produced by FastQC (version 0.11.5)