FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160935
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA32140.2768458182413313No Hit
CTCCGGGCCCATTAAACTTGGGGGTAGCTAAACTGAAACTTTATCAGACA28520.2456640552657987No Hit
CCGAGCCCACGAGACACCGCTGTCTCTTATACACATCTGACGCTGCCGAC23000.19811617360145053No Hit
GTACAACAGTACATTTATGTATATCGTACATTAAACTATTTTCCCCAAGC15560.13402989831472048No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13410.115510342956324No Hit
GTTTATGACTGTATGGTGTATGTCAGATAACACAGATATGTCCTTATAAC13390.11533806802275753No Hit
GTGTATGTCAGATAACACAGATATGTCCTTATAACATTAGTTTAATGTGT13370.11516579308919103No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC12880.1109450572168123No Hit
GCATATAAGCTAGTACATTAAATCAATGGTTCAGGTCATAAAATAATCAT12750.10982527014863021No Hit
GGACAGCACACAGTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCA11780.10146993587065599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT4150.053.97543768
CGAGACA4500.050.55294410
CGCGAGT352.0881293E-549.99741415
TCGGTGG4650.048.9243170
ATCTCGG4700.048.4038467
CTCGGTG4750.047.89432569
GATCTCG4700.047.65916466
ACGAGAC5150.044.8520479
CGTCTAG5850.044.3418771
CCACGAG5450.043.0252957
GTAGATC5400.042.77556663
CGAGGAT5300.042.26196750
GCCCACG5600.041.8728335
CACCGCT5600.041.87283315
ACACCGC5350.041.21282614
CCGCTGT6100.040.16185817
ACCGCTG5950.039.99793616
GACACCG6000.039.66461613
TTGACGG6750.038.36838531
GACGGCT6800.038.0862733
Produced by FastQC (version 0.11.5)