FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25352897
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA28100.030.51324762
CGCCGTA28700.028.90107559
CGTCTAG62750.028.4280091
GCCGTAT32250.026.15256560
CCGTATC34750.024.57321461
ACGGCTA70950.023.5303834
CGGCTAT72700.023.1557635
TTGACGG72250.023.1075931
CCGACGA511200.021.8188170
TCGCCGT39400.021.14121658
TAGACTG148000.021.0682895
CTAGACT142500.019.770494
GACGGCT85550.019.63760433
TGACGGC86650.019.50971632
GCCGACG720500.019.4296970
GTGGTCG44350.019.09714354
CGACGAA354450.017.86172570
TCTAGAC156600.017.4566443
GTATAGG54450.016.9277921
GATCTCG54150.016.7395847
Produced by FastQC (version 0.11.5)