FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25741895
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA696710.2706521800356967No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC366650.14243318139554217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG141100.039.6645051
GACGGCT175400.030.84976833
ACGGCTA181350.030.01095234
TTGACGG180700.029.53840831
TAGACTG227250.029.4598085
CTAGACT223450.029.0067924
CGGCTAT192800.028.46327435
TGACGGC191300.028.2858932
CTAGACG101300.028.0913983
GACTGTG231150.027.3749757
CTTGACG203450.026.21844130
CCTACGG106250.025.9586412
TAGACGC127450.025.3455094
ACTGTGT260050.025.3021748
GACGCAC113100.024.9713736
GTCCGCA112800.024.57488332
CTACGGT111800.024.51355413
ACCTACG115850.023.89810411
AAGATAC215500.023.72672767
AAAGTAG226750.023.49195549
Produced by FastQC (version 0.11.5)