FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591049
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCCCACGAGACACCGCTGTCTCTTATACACATCTGACGCTGCCGAC26830.4539386751352257No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC11350.19203145593681742No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT10670.18052648765161602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7100.1201254041543087No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA6510.11014315225979572No Hit
CCTTAGACGCTTCATGATCTAACAACTTACTATGGTTGGCATGCATAATA6040.10219118888620063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAG4600.067.715357
CGAGACA4500.067.6646210
CGAGCCC4500.064.5973362
ACCGCTG4300.064.30038516
GCCCACG4850.063.503245
CCGAGCC4600.063.257321
TCGGTGG4800.061.2481570
ACGTGTA4400.061.24814659
ACGTCCG401.0662916E-861.2481465
ATACCGG401.0662916E-861.24814637
CACCGCT4600.060.86772515
TCGGGGA352.9741022E-759.9981888
CTCGGTG4900.059.99818869
CGTACGC352.9741022E-759.99818833
CGAGATA4850.059.895150
ATCTCGG4850.059.1734767
GGACGCA308.292887E-658.4304581
GATCTCG5000.058.09824466
TCTCGGT5100.057.6453268
ACGAGAT4900.057.14113249