FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283164
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20100.034.3464781
CGACGAA226450.024.41916370
CCGACGA345200.024.20068470
TTGACGG29100.023.93511631
TAGGGCG25950.021.58032831
CGGCTAT31650.021.3421335
ACGGCTA31300.020.01563634
GCCGACG477800.019.5427570
GTATAGG42200.019.4319081
TGACGGC38150.018.53228432
CTATACT76700.018.1604064
GACGAAG153000.017.5906170
CGTACTC9700.017.3211213
TAGACTG73400.017.2119875
GACGGCT36500.017.16428833
GGGCGTT30950.017.07583433
TCTAGAC74350.016.6659013
CTAGACG17950.016.5752853
CTAGACT72450.016.3756714
TAGACGC31450.016.3581624
Produced by FastQC (version 0.11.5)