FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701090
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG2750.038.238061
ATCGTGT2700.029.81395558
GACGGCT3650.026.8490433
TCGGTGG3550.024.64717970
CGGCTAT4150.024.45757935
TCTCGGT3450.023.33222868
GATCTCG3500.022.99848766
CGAGACA3350.022.98441110
TACATCG3400.022.64640655
TTGACGG4350.022.52850331
TGACGGC4550.022.30746332
GCTCGAA3350.021.94007111
CGCGGTC2900.021.72391533
ACGGCTA4800.021.14561734
TCATACG3150.021.11050238
CTCGGTG4000.020.99900469
CGGTTAG4250.020.58764356
CCACGAG4250.020.5872617
CGAGCCC3800.020.2760872
GCCCACG4000.020.1240465
Produced by FastQC (version 0.11.5)