FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9499732
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG13650.031.0607381
TTGACGG15050.025.58175531
ACGGCTA16300.024.47835434
CGGCTAT16550.023.68488535
TAGACTG49200.022.9757865
TATACTG29250.020.2205895
TGACGGC20000.019.95017432
CCGACGA49400.019.41159270
CTTGACG20200.019.40621230
CTAGACT50750.019.3093954
TCTAGAC47800.019.2587073
TACACTG55650.018.4261865
TGCCGAC94550.018.21130670
ACGACAT22900.017.72801270
GTATTAG21200.017.6850781
CTATACT37950.017.5221944
CGACGAC36050.017.2803470
GACGGCT22500.017.26654433
GTATAGG21950.017.0808051
CTACACT47250.016.8880584
Produced by FastQC (version 0.11.5)