FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24747943
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32500.025.554091
TTGACGG32400.022.0371131
CGAGAGT255650.020.75387670
CGGCTAT35850.020.50153735
ACGGCTA35750.020.26551434
ACGAGAG360000.020.12396870
TAGACTG127800.019.85375
AGAGTCG137050.019.33136770
CTACACT126200.018.9412234
CTAGACT123750.018.8637184
CTATACT88800.018.7209244
TACACTG147600.018.5656345
TATACTG75250.018.3241625
CCTATAC71150.018.2023683
TAGGCCG37450.017.8490835
CTTGACG43100.017.1346630
GAGTCGG102800.017.09056370
TCTAGAC121850.017.063263
TACACCG27900.016.5578565
GCCGACC59050.016.47671770
Produced by FastQC (version 0.11.5)