FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112529
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG16760.1506477583955115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGA900.070.08991
CCGTAAA1450.070.039452
TCGTCGG455.456968E-1270.00796518
TCGCAAG1000.070.0079612
AAATCCG301.0234544E-770.0079626
GATCGGA500.070.0079619
CGTTCTA500.070.0079626
CATTCGG150.002223882170.0079628
AACGGCG550.070.0079619
TCTCGAC150.002223882170.0079622
GTCGTCG252.839015E-670.0079620
ATGCGCG850.070.0079612
CGAGAAT353.7107384E-970.0079623
TTCGCGT550.070.0079621
TTCGCGA550.070.0079626
TGGCTCG353.7107384E-970.0079628
ATACCGT500.070.0079624
ATCGAAT301.0234544E-770.0079620
TACCGAT500.070.0079623
TCCGGAA353.7107384E-970.00481430
Produced by FastQC (version 0.11.5)