FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8826239
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA6600.038.70958759
TCGCCGT6650.037.36639858
CGCGTAA708.7143053E-734.99711269
GCCGTAT7650.032.93808760
CGTATCA8650.030.74890962
CCGTATC9450.028.1456761
TATGGCG14250.027.7560316
ATTTCGT9500.027.26137242
TCGTCAA11200.027.1849560
GTCCTAC42250.027.0421451
TACGACT4200.026.6641654
TATACTG44950.026.1599485
TCGACGC1508.8584784E-1025.66498659
CGTCAAG11500.025.56296561
TGGTCGC10400.025.2385955
GTCGCCG10000.025.19863557
TTCGTCA10300.025.1438444
AAGTCGT13700.024.7796857
CTATACT38600.024.7515624
GTAGGAC42000.024.5833933
Produced by FastQC (version 0.11.5)