FastQCFastQC Report
Tue 14 Jul 2020
HW5TCBGXF_n02_dpr149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5TCBGXF_n02_dpr149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218866
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGT6400.030.62339642
TCGTCAA5400.030.46100860
GATTTCG5950.029.9984341
TTTCGTC6200.029.91778843
CGACGTA500.007214361327.99853541
CGTCATT6450.027.673346
GTCCTAC18450.027.5422231
TCACGTT9050.026.29883627
TTCGTCA7150.025.942744
AAGTCGT6100.025.8183257
CGTCAAG6700.025.07301761
CGAAAAA6300.024.44490225
ACGTTTT9200.024.34799629
CGTTTTT9250.024.21638530
TCGTCAT7450.023.95876145
TACACTG37150.023.9284785
AGTCGTC6450.023.8747258
GTCGTCA6500.023.69106759
CTACCCT30550.023.3705774
CACGTTT9900.022.97995828
Produced by FastQC (version 0.11.5)